Pathogenicity of Nosema sp. (Microsporidia) in the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)
نویسندگان
چکیده
Biological control using pathogenic microsporidia could be an alternative to chemical control of the diamondback moth (DBM) Plutella xylostella (Lepidoptera: Plutellidae). The microsporidium Nosema bombycis (NB) is one of the numerous pathogens that can be used in the Integrated Pest Management (IPM) of DBM. However, its pathogenicity or effectiveness can be influenced by various factors, particularly temperature. This study was therefore conducted to investigate the effect of temperature on NB infection of DBM larvae. Second-instar larvae at different doses (spore concentration: 0, 1×10²,1×10³,1×10⁴, and 1×10⁵) at 15°, 20°, 25°, 30° and 35°C and a relative humidity(RH) of 65% and light dark cycle (L:D) of 12∶12. Larval mortality was recorded at 24 h intervals until the larvae had either died or pupated. The results showed that the spore concentration had a significant negative effect on larval survival at all temperatures, although this effect was more pronounced (92%) at 35°C compared with that at 20 and 30°C (≃50%) and 25°C (26%). Histological observations showed that Nosema preferentially infected the adipose tissue and epithelial cells of the midgut, resulting in marked vacuolization of the cytoplasm. These findings suggest that Nosema damaged the midgut epithelial cells. Our results suggest that Nosema had a direct adverse effect on DBM, and could be utilized as an important biopesticide alternative to chemical insecticides in IPM.
منابع مشابه
Feeding and oviposition preferences of the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) on six Brassicaceae host plant species
متن کامل
Demographic parameters of the diamondback moth, Plutella xylostella (Lep.: Plutellidae) on five rapeseed cultivars
شبپرهی پشت الماسی، (Plutella xylostella (L.) (Lep.:Plutellidae، یکی از مخربترین آفات گیاهان چلیپیان در سراسر دنیا است. در این تحقیق پارامترهای دموگرافیک این حشره روی پنج رقم کلزا شامل لیکورد، مودنا، اکاپی، RGsoo3و REGXkobra بررسی شد. آزمایشها در اطاقک رشد با دمای 1±25 درجهی سانتیگراد، رطوبت نسبی 5±60 درصد و دورهنوری 16 ساعت روشنایی و 8 ساعت تاریکی انجام شد. مرگ و میر مراحل قبل از بل...
متن کاملChronic Sublethal Effects of Cantharidin on the Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae)
The diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is a major pest of cruciferous vegetables worldwide. Cantharidin, a natural toxin isolated from blister beetles, has been reported to be toxic to P. xylostella. However, little is known on the chronic sublethal effects of cantharidin on this species. In this study, we assessed the changes of susceptibility, develop...
متن کامل(A new record for Iran of Dolichogenidea appellator (Hym.: Braconidae: Microgastrinae), a larval endoparasitoid of diamondback moth, Plutella xylostella (Lep.: Plutellidae
طی مطالعاتی که در سال 1390 روی بید کلم، Plutella xylostella (L.)، در استان اصفهان انجام شد، یک گونه زنبور پارازیتوئید به نام Dolichogenidea appellator (Telenga) از پرورش لاروهای بید کلم جمعآوریشده از مزارع کلمپیچ، واقع در مرکز تحقیقات کشاورزی و منابع طبیعی استان اصفهان بهدست آمد که برای فون ایران جدید است. این زنبور، علاوهبر بید کلم، بالپولکداران دیگری از خانواده Gelechiidae، نظیر Scrobi...
متن کاملHORTICULTURAL ENTOMOLOGY Evaluating Trap Crops for Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)
Potential trap crops for the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), were evaluated through a series of ovipositional preference and larval survival experiments in outdoor screenhouses in 2002 and 2003. Hosts examined as trap crops were glossy and waxy collards, Brassica oleracea L. variety acephala; Indian mustard, Brassica juncea (L.) Czern; and yellow rocket,Ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 8 شماره
صفحات -
تاریخ انتشار 2013